African Champions League 2016
1st round / 1st leg
2015 Champions: TP Mazembe
| 45 | 13 Mar 2016 - 4 p.m. | |
 AS Vita Club |
1 : 0 | Ferroviário Maputo  |
| 46 | 13 Mar 2016 - 4 p.m. | |
 mamelodi-sundowns |
2 : 0 | AC Leopards  |
| 47 | 12 Mar 2016 - 4 p.m. | |
 Warri Wolves |
0 : 1 | Al Merreikh  |
| 48 | 13 Mar 2016 - 4 p.m. | |
| Étoile du Congo |
1 : 1 | Entente Setif  |
| 49 | 12 Mar 2016 - 5 p.m. | |
 Wydad Casablanca |
5 : 1 | CNaPS Sport  |
| 50 | 13 Mar 2016 - 3 p.m. | |
 Saint George |
2 : 2 | TP Mazembe |
| 51 | 12 Mar 2016 - 4 p.m. | |
| Enyimba |
5 : 1 | Vital'O  |
| 52 | 13 Mar 2016 - 4 p.m. | |
| Olympique Khouribga |
1 : 1 | Etoile du Sahel  |
| 53 | 12 Mar 2016 - 5 p.m. | |
 Stade Malien |
2 : 0 | Coton Sport  |
| 54 | 13 Mar 2016 - 4 p.m. | |
 ZESCO United |
4 : 1 | Horoya  |
| 55 | 12 Mar 2016 - 4 p.m. | |
 APR |
1 : 2 | Young Africans  |
| 56 | 13 Mar 2016 - 3 p.m. | |
 Recreativo do Libolo |
0 : 0 | Al Ahly SC  |
| 57 | 13 Mar 2016 - 4 p.m. | |
| Kaizer Chiefs |
0 : 1 | ASEC Mimosas  |
| 58 | 12 Mar 2016 - 4 p.m. | |
 Al Ahli Tripoli |
1 : 0 | Al Hilal |
| 60 | 13 Mar 2016 - 4 p.m. | |
| Union Douala |
0 : 1 | Zamalek |
| 61 | 12 Mar 2016 - 5 p.m. | |
| Club Africain |
1 : 0 | Mouloudia Béjaïa |